ELISA principals and applications for professionals

Course Overview

• Course Title: ELISA principals and applications for professionals
• Instructor: Mohamed AL ashram
• Target Audience:
  • Researchers in immunology, microbiology, and biotechnology
  • Laboratory technicians and analysts in food safety, environmental, and clinical settings
  • Quality control personnel in the pharmaceutical and food industries
  • Medical professionals seeking a deeper understanding of diagnostic tools
• Prerequisites: Basic understanding of biological principles (antigens, antibodies, immune system)

Curriculum Highlights

• Key Topics Covered:
  • ELISA Fundamentals
    • Theoretical underpinnings of antigen-antibody interactions
    • Various types of ELISA assays (direct, indirect, sandwich)
    • Components and functions of ELISA kits
  • Advanced Techniques
    • Double antibody sandwich ELISA for enhanced specificity
    • Competitive ELISA for quantifying small molecules
    • Understanding factors affecting ELISA performance (sensitivity, specificity)
  • Applications in Diverse Fields
    • Immunodiagnostics of infectious diseases (e.g., COVID-19 testing)
    • Food safety analysis (detecting foodborne pathogens)
    • Environmental monitoring (assessing pollutant levels)
    • Allergen detection in food products
    • Autoimmune disease diagnostics
  • Data Analysis and Interpretation
    • Principles of spectrophotometry and result interpretation
    • Statistical analysis of ELISA data
    • Quality control and validation procedures
• Key Skills Learned:
  • Basic immunology
  • Monoclonal antibody
  • Different types of antigens
  • Different methods for antigen detection
  • Common diagnostic techniques
  • Enzyme immunoassay
  • Mycotoxin and mycotoxicosis
  • Food hormones residues

Course Format

• Duration: 2.5 hours on-demand video
• Format: Self-paced online course
• Resources:
  • 36 downloadable resources
  • Access on mobile and TV
  • Certificate of completion